What cells should be examined during FISH testing?

[Music] what cell should be examined during fish testing fish stands for fluorescence in situ hybridization and is a test that map's the genetic material in a person cells so when you take all the cells from the bone marrow the myeloma cells might only be 10% or 15% to 20% in there so it's important that we just look at the myeloma cells to identify the abnormalities that are relevant there are two ways of doing it one is to what we call enrich the cell so we use a magnetic bead which has antibodies against some myeloma markers which will allow us to concentrate the myeloma cells so that we are just looking at the myeloma cells another way to do that is what we call a cytoplasmic immunoglobulin facial cig fish do you basically identify the plasma cells by using additional markers so that we only looking at the plasma cells when you're looking at it under this slide to see if they have a specific fish of normality